Nuance™ Resources
Brochures
- Nuance Brochure (pdf)
- Nuance TRIO Brochure (pdf)
- Nuance 2.4 brochure (pdf)
White Papers
- Measuring double-positivity in individual tumor cells in FFPE tissue sections (pdf)
- Multiplexing with Multispectral Imaging: From Mice to Microscopy (pdf)
- Autofluorescence removal, multiplexing and automated analysis methods for in-vivo fluorescence imaging (pdf)
Application & Technical Notes
- Analyzing signaling pathway activity in FFPE tissue sections for cancer research and drug development (pdf)
- Detecting phenotypal subgroups in breast cancer using multiplexed protein expression analysis in intact tissue sections (pdf)
- Quantitating protein expression in intact tissue sections for cancer research (pdf)
- In situ miRNA Expression Profiling Using Multispectral Imaging to Elucidate Pathways Involved in Neurodegenerative Disease (pdf)
- Detection of Multiple QDots in Brain Tissue (pdf)
- Rescue of overstained specimens (pdf)
- Separation of multiple stains which co-localize in a specimen (pdf)
- Spectral Imaging for Separation of Fluorescent Signals from Autofluorescence (pdf)
User Protocols
Publications
- Methods: Cellular context in epigenetics: Quantitative multicolor imaging and automated per-cell analysis of miRNAs and their putative targets (link to publisher page)
- Innovations in Pharmaceutical Technology (June 2010): The Quantification of Multiple Signaling Pathway Proteins in Intact Tissue Sections (pdf)
- Fluorescence and Brightfield Microscopy References (pdf)
- Quantitating signaling pathway proteins in FFPE sections using multispectral imaging and automated image analysis (pdf)
- Automated assessment of ER and ki67 in individual tumor cells in intact tissue sections using multicolor IHC, multispectral imaging, and pattern-recognition-based image analysis software (pdf)
- Detecting Phenotypical Subtypes of Breast Cancer with Multiplexed Immunohistochemistry (pdf)
- Co-localization of a miRNA and its Putative Target using Multispectral Imaging (pdf)
- The Reduction or Elimination of Tissue Autofluroescence in Formalin-Fixed, Paraffin-Embedded Renal Biopsy Images Using Spectral Imaging (pdf)
- Current Protocols in Molecular Biology 2008 Visualization of microscopy-based spectral imaging data from multi-label tissue sections (pdf)
- BioTechniques Application Forum: In Situ miRNA Expression Profiling Using Multispectral Imaging to Elucidate Pathways Involved in Neurodegenerative Disease (pdf)
- Cytometry Part A: Multispectral Imaging in Biology and Medicine: Slices of Life (pdf)
- LabMed: Spectral Imaging and Pathology: Seeing More (pdf)
- Expert Opinion on Medical Diagnostics: Multispectral Imaging and Pathology: Seeing and Doing More (pdf)
- Tissue Cytometry Platform For Quantitating Multiple Signaling Parthways Proteins In Intact Tissue Sections (pdf)
Other
- New method to detect EGFR activation in FFPE tissue sections (wmv, 56 MB)
Learn about a new integrated approach to assess EGFR activation in formalin-fixed, paraffin-embedded (FFPE) tissue sections by measuring sub-cellular localization of phospho-EGFR (pEGFR), total EGFR, and a downstream kinase, phospho-ERK (pERK). Panelists will discuss how to combine commercially developed antibody cocktails with spectral imaging and automated image analysis to accurately and easily determine percent activation of EGFR-positive cells using the detection of pEGFR and pERK as indicators of activation. - Randall K. Wetzel, Director, Cytometry, Cell Signaling Technology - James R. Mansfield, Director, Multispectral Imaging Systems, CRi. November 23, 2010. - Nuance Tips & Tricks Webinar (wmv, 32.1 MB)
- Quantitating multiple protein expressions in intact tissue sections (wmv, 73 MB)
Recording of free webinar by James Mansfield, Director of Multispectral Imaging Systems, providing an overview of how leading scientists are using multi-label imaging in combination with pattern-recognition image analysis software to analyze biomarkers specific to important disease-related cellular states, such as signaling configuration, stem cell pluripotency, and receptor expression. June 17, 2010.
