Multimedia
inForm
inForm software: overview of inForm 1.0 (mov, 165 MB)
Movie showing the new Nuance 1.0 interface with workflow-oriented interface. Demonstration of quantitation of multiple molecular markers (ER/PR and HER2), utilization of spectral libraries, learn-by-example interface, speed, accuracy, batch-processing, scoring, and data export.
Quantitating multiple protein expressions in intact tissue sections (wmv, 73 MB)
Recording of free webinar by James Mansfield, Director of Multispectral Imaging Systems, providing an overview of how leading scientists are using multi-label imaging in combination with pattern-recognition image analysis software to analyze biomarkers specific to important disease-related cellular states, such as signaling configuration, stem cell pluripotency, and receptor expression. June 17, 2010.
Vectra
Vectra imaging system (mov, 116 MB)
Movie showing the new Vectra imaging system, the world's only multispectral fluorescence- and brightfield-capable microscope slide analysis system. Comes with inForm for quantitating multiple molecular markers, a learn-by-example interface, speed, accuracy, batch-processing, scoring, and data export.
Quantitating multiple protein expressions in intact tissue sections (wmv, 73 MB)
Recording of free webinar by James Mansfield, Director of Multispectral Imaging Systems, providing an overview of how leading scientists are using multi-label imaging in combination with pattern-recognition image analysis software to analyze biomarkers specific to important disease-related cellular states, such as signaling configuration, stem cell pluripotency, and receptor expression. June 17, 2010.
Maestro
Movie of DyCE time series (gif, 1.5 MB)
Time sequence of Dynamic Contract Enhancement (DyCE) acquisition. The final DyCE image shows each organ in pseudocolor (heart = red, gut = blue, lungs = yellow, liver = cyan, brain = white, etc).
Courtesy Elizabeth Hillman, Columbia University, New York, NY
Maestro software: RCA demonstration (avi, 257.0 MB)
Qdot, food, and autofluorescence signals are separated from each other using the Real Component Analysis (RCA) tool in CRi's Maestro software.
Sample courtesy Shuming Nie, Emory University, Atlanta, GA
DyCE demonstration (avi, 136.6 MB)
Movie demonstrating the new Dynamic Contrast Enhancement (DyCE) tool available in CRi's Maestro software. The MultiView platform is shown, along with display options, and a comparison to a CT mouse atlas image.
Sample courtesy Elizabeth Hillman, Columbia University, New York, NY
Maestro software: how to unmix a spectral cube (gif, 1.4 MB)
FITC, Cy3.5, TRITC, and autofluorescence signals are cleanly separated and then displayed in a Maestro unmixed image.
Sample and images courtesy of R. Weissleder, U. Mahmood and J. Tam, MGH, Boston, MA
Maestro spectral image series of unmixed data (gif, 507.5 KB)
Spectral imaging enables multiplexing, even in the near-infrared. Here we see four signals, AF680 (green). AF700 (blue), AF750 (red), and Matrigel (gray), along with combinations of two (cyan) and three (pink) markers.
Sample courtesy Stanford University Workshop
Nuance
Nuance Tips & Tricks Webinar (wmv, 32.1 MB)
Recording of free webinar for existing Nuance users or those considering the purchase of a Nuance system. February 17, 2010.
Nuance software: how to unmix a spectral cube (avi, 219.4 MB)
Movie showing the Nuance spectral unmixing tools available for immunohistochemistry, in this case separating spectrally and spatially overlapping hematoxylin and DAB signals.
Nuance software: RCA demonstration (avi, 340.0 MB)
Movie of the Nuance Real Component Analysis (RCA) spectral unmixing tool available for fluorescence microscopy, in this case separating two qdot signals from autofluorescence.
Nuance Chromogenic spectral image series of unmixed data (gif, 7.0 MB)
Multiple signals, including hematoxylin, ki67, pHH3, and CC3, unmixed using CRi's Nuance system. Note the options available for displaying different combinations of signals in pseudocolor.
Images courtesy Chris Kerfoot, Mosaic Labs
Nuance Fluorescence spectral image series of unmixed data (gif, 9.4 MB)
Six signals (DAPI + Ki67 + CD3 + CD20 + IgD + CD68) separated and displayed independently and in unmixed Nuance pseudocolor.
Sample courtesy Stefania Pittaluga, NCI
Quantitating multiple protein expressions in intact tissue sections (wmv, 73 MB)
Recording of free webinar by James Mansfield, Director of Multispectral Imaging Systems, providing an overview of how leading scientists are using multi-label imaging in combination with pattern-recognition image analysis software to analyze biomarkers specific to important disease-related cellular states, such as signaling configuration, stem cell pluripotency, and receptor expression. June 17, 2010.
Oosight
Movie of Enucleation of Rhesus Monkey Oocyte (avi, 10.6 MB)
Screencapture of Oosight system being used for enucleation of rhesus monkey oocytes. Note the high-contrast Live Oosight mode, versus conventional imaging.
Sample courtesy Shoukhrat Mitalipov, Oregon National Primate Research Center, OHSU
Abrio
Movie of a living growth cone (avi, 18.4 MB)
Time-lapse retardance magnitude movie of the architectural dynamics of fine structure in the growth cone cytoskeleton of an Aplysia bag cell neuron.
Courtesy Kaoru Katoh, Katherine Hammer, Peter J.S. Smith, Rudolf Oldenbourg; MBL, Woods Hole, MA
Moving of a crane fly spermatocyte undergoing Meiosis (avi, 134.7 MB)
Time-lapse retardance magnitude movie showing meiosis I of the spermatocyte of the crane fly (Nephrotoma suturalis).
Courtesy James LaFountain, University at Buffalo, Buffalo, NY and Rudolf Oldenbourg, MBL, Woods Hole, MA
