inForm™ Resources
Brochures
- inForm Brochure (pdf)
White Papers
Application & Technical Notes
- Analyzing signaling pathway activity in FFPE tissue sections for cancer research and drug development (pdf)
- Detecting phenotypal subgroups in breast cancer using multiplexed protein expression analysis in intact tissue sections (pdf)
- Quantitating protein expression in intact tissue sections for cancer research (pdf)
Publications
- Methods: Cellular context in epigenetics: Quantitative multicolor imaging and automated per-cell analysis of miRNAs and their putative targets (link to publisher page)
- Innovations in Pharmaceutical Technology (June 2010): The Quantification of Multiple Signaling Pathway Proteins in Intact Tissue Sections (pdf)
- Fluorescence and Brightfield Microscopy References (pdf)
- Quantitating signaling pathway proteins in FFPE sections using multispectral imaging and automated image analysis (pdf)
- Automated assessment of ER and ki67 in individual tumor cells in intact tissue sections using multicolor IHC, multispectral imaging, and pattern-recognition-based image analysis software (pdf)
- Detecting Phenotypical Subtypes of Breast Cancer with Multiplexed Immunohistochemistry (pdf)
- Tissue Cytometry Platform For Quantitating Multiple Signaling Pathways Proteins In Intact Tissue Sections (pdf)
- AACR Poster 2008: Molecular epidemiology of clinical tissues with multi-parameter IHC (pdf)
- USCAP Poster 2008: Automated Quantitative Assessment of Multiple Immunohistochemical Stains in Cancer Tissue Sections in Preclinical and Clinical Trials (pdf)
- San Antonio Breast Cancer Symposium 2007: Multiplexed immunohistochemical quantitative molecular phenotyping via multispectral imaging and automated segmentation (pdf)
Other
- New method to detect EGFR activation in FFPE tissue sections (wmv, 56 MB)
Learn about a new integrated approach to assess EGFR activation in formalin-fixed, paraffin-embedded (FFPE) tissue sections by measuring sub-cellular localization of phospho-EGFR (pEGFR), total EGFR, and a downstream kinase, phospho-ERK (pERK). Panelists will discuss how to combine commercially developed antibody cocktails with spectral imaging and automated image analysis to accurately and easily determine percent activation of EGFR-positive cells using the detection of pEGFR and pERK as indicators of activation. - Randall K. Wetzel, Director, Cytometry, Cell Signaling Technology - James R. Mansfield, Director, Multispectral Imaging Systems, CRi. November 23, 2010. - Quantitating multiple protein expressions in intact tissue sections (wmv, 73 MB)
Recording of free webinar by James Mansfield, Director of Multispectral Imaging Systems, providing an overview of how leading scientists are using multi-label imaging in combination with pattern-recognition image analysis software to analyze biomarkers specific to important disease-related cellular states, such as signaling configuration, stem cell pluripotency, and receptor expression. June 17, 2010.
