Contact | Sales | Support | Partners | Careers
Features & Benefits Applications Support Literature References Software Downloads Browse Imaging Gallery

NEW: Stunning Detail in Human MI and MII Oocyte Images Seen in New Downloadable PDF

Image of Oosight

Non-invasively reveal the spindle and zona pellucida in living oocytes

The CRi Oosight™ Imaging System is a next-generation, improved version of CRi's own, original, industry-leading SpindleView™ system. Use the system for assisted reproductive technology applications for imaging the meiotic and mitotic spindle, the three-layered zona pellucida, and other birefringent structures. Assess the quality of living oocytes. Non-invasively locate and position the spindle for ICSI. Monitor changes in spindle parameters from oocyte to oocyte. Remove the spindle in nuclear transfer techniques without using Hoechst dye DNA staining, thus minimizing damage to vital cytoplasmic organelles. Track the changing molecular organization of the zona pellucida through embryo development.

An Oosight image and a Hoffman Modulation Contrast image

Oosight image of an oocyte prior to injection, spindle is identified by the white arrow.

Hoffman Modulation Contrast (HMC) image of an oocyte during injection. Note: no spindle is visible.

NEW: Stunning Detail in Human MI and MII Oocyte Images Seen in New Downloadable PDF

Oosight pseudocolor image showing outstanding detail of spindle structure.

This Downloadable PDF Image Gallery (10 MB) shows stunning detail of the meiotic spindle and trilaminar zona pellucida both in MI anaphase/telophase oocytes as well as in MII oocytes. Images courtesy of RMANJ and RBA, Atlanta.

Movie of the enucleation of a Rhesus monkey oocyte, using CRi's Oosight system

AVI movie of Oosight system being used to enucleate a Rhesus monkey oocyte

Click here to download the movie. Be patient if you do not have a broadband Internet connection, since it is of high quality and large (10.8 MB). Windows users should be able to play the movie using the latest version of Microsoft's MediaPlayer. If your browser will not play the movie directly, right-click on the link and choose Save Target As to save the movie to your desktop. Then try opening the AVI file with your movie player of choice. Notice how easy to use the Oosight software is, and how clearly the spindle appears in the Live Oosight view. This movie was acquired in real-time using third-party screen-capture software. Oosight movies can be exported directly from CRi's software, but this particular movie shows the entire program interface.

The multi-step process of cloning is very inefficient, with yields of a few percent or less. In the BioTechniques® 2007 Protocol Guide, we describe the use of the Oosight system to improve enucleation precision and to assess oocyte quality to increase overall cloning success. Click here to download the full protocol (.pdf).

System Details

Format

Components

To find out more or to obtain a sales quote, call US Toll-Free 1-800-383-7924, send e-mail to oosight@cri-inc.com, or click here to fill out a short form to request that your local sales representative contact you immediately.