Oosight™
Revealing critical structures in living unstained oocytes
The Oosight™ imaging system utilizes CRi’s own core LC-PolScope™ technology, offering the unique capability of providing high-contrast quantitative images of the spindle apparatus and tri-laminar zona pellucida without the use of exogenous dyes or labels. Oosight systems have applications in both in vitro fertilization (IVF) and general-purpose research laboratories. The Oosight system is an easy add-on to existing microscope workstations, and is compatible with most third-party laser systems. The Oosight system has also been shown to be a critical enabling technology for somatic cell nuclear transfer (SCNT).
Series of Human oocytes and Embryos: first in series shows a strongly birefringent, normal barrel-shaped spindle. The second image is of a spindle-negative oocyte. The third image is of an immature human oocyte in Meiosis I showing an extruded polar body with a bisecting prominent MI spindle. Next image series displays the Oosight system's ability to find and define spindle and inner zona retardance metrics. Finally, a normal versus abnormal embryo is shown.
Oocyte images courtesy RMA NJ.Embryo images courtesy D Hlinka, Pronatal, Czech Republic.
The Oosight™ system comes in two models:
| Oosight Spindle-Check | Oosight Meta | |
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| LC Compensator |  |
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| CPIF | |
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| CCD Camera | |
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| C-Mount for Camera | |
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| Image Capture Software | |
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| Automated Spindle Finder™ | |
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| Automated Zona Finder™ | |
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| Reporting Tools | |
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| Movie Capture | |
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| *The Spindle And Zona Finder are tools in the Oosight software used to automatically find and report on spindle and zona metrics, including mean retardance and orientation offset. | ||
| NOTE: The Oosight system is compatible with most research-grade microscopes and laser systems. Glass bottom dishes are required. | ||
