Nuance™
Multi-label imaging without cross-talk
As biological imaging becomes more about studying complex systems rather than single events, Nuance multispectral imaging systems let you quantitate multiple molecular markers even when they are co-localized in a single tissue section, producing clear and accurate images of each individual label on a multi-label tissue section.
The unique and patented Real Component Analysis (RCA) tool automatically detects spectral components. View and quantitate signals as either single images, as overlays, or as a composite image showing all unmixed labels in high contrast. Nuance systems offer powerful spectral unmixing capability to remove autofluorescence, thereby dramatically increasing signal-to-noise and improving the accuracy of your results.
View the software clips to learn more about how Nuance captures, unmixes and quantitates. Nuance systems are now compatible with Molecular Devices' MetaMorph™. Nuance systems deliver flow-cytometry-like data while preserving the morphological context down to the sub-cellular level.
Brightfield immunohistochemistry with color re-mapping for legibility. Oral squamous carcinoma (FFPE, 20X) stained for CD34 (endothelium, Nova Red) and podoplanin (VIP, purple), and counterstained with hematoxylin. The spectral dataset was unmixed, the component planes re-colored (purple to green) and displayed as a composite image with improved contrast and legibility over the original.
Sample courtesy Michael Feldman, University of Pennsylvania.
There are two Nuance models:
| Nuance EX | Nuance FX | ||
|---|---|---|---|
| Bandwidth Flexibility | 20 and 40 nm | 20 and 40 nm | |
| Scientific-Grade CCD | Cooled Sony ICX285 | Cooled Sony ICX285 | |
| Mount Required* | 1x C-mount | 1x C-mount | |
| Acquisition and Analysis Software |  |
|
|
| Applications | |||
| Brightfield | |
||
| Fluorescence | |
|
|
| NIR Fluorescence | |
|
|
| *An F-mount macro lens is available for macroscopic applications. | |||
Nuance systems can be mounted on any microscope with a standard C-mount and offer CRi’s patented multispectral imaging capability at the sub-cellular, cellular and tissue level. Nuance’s powerful combination of multispectral hardware and sophisticated data analysis can dramatically improve tissue-based imaging.
