Maestro 2
Fully automated multispectral small-animal imaging
The Maestro 2 in vivo imaging system sets a new standard for all-optical imaging of small animals. It is fully automated for consistent results and ease-of-use, ideal for non-technical personnel, and for high-throughput applications. The Maestro 2, like all Maestro systems, also offers the capability to generate anatomic organ maps and to anatomically target co-localization using CRi’s new all-optical anatomic imaging breakthrough, DyCE.
The Maestro 2 system comes equipped with a light-tight enclosure, configurable imaging area, tunable multispectral camera system, heated imaging chamber, computer and pre-installed easy-to-use software on a powerful computer workstation. The hardware is complemented by industry-leading algorithms for accurate spectral unmixing. These systems contain an optimized, solid-state LCTF-based multispectral imaging system and excitation source that can capture high-spectral-resolution multispectral fluorescence images of small animals at multiple, user-defined wavelengths ranging from the blue out to the near infrared. Also included is a Xenon-based excitation light source with motorized filter-selectable spectral ranges, and a heated stage with a triple nose-cone anesthesia manifold.
In order to illustrate the high sensitivity of the Maestro system, images and data were acquired from 4 mice with varying amounts of doxorubicin (DOX) injected. The Maestro system shows a significant increase in sensitivity compared with conventional monochrome imaging.
