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Chromogenic Kits

The following chromogenic kits have been validated for use with the Nuance multispectral imaging systems:

The Biocare Medical Double Vision Multiple Stains:
Stains for multiple antibodies on a single slide

Vector Laboratories ProtOn Biotin Labeling Kit:
ProtOn Biotin Labeling Kit, Cat. No. PLK-1202

Reference the following guide on multi-label staining:
User Protocol: Practical Multiple Staining Pamphlet

ER_ki67_Unmixed_Composite_all ER_ki67_Unmixed_Composite_ER ER_ki67_Unmixed_Composite_hem ER_ki67_Unmixed_Composite_inverted ER_ki67_Unmixed_Composite_ki67
+ Click on images to enlarge

Figures: Nuance images of slide stained with Biocare Medical Double Vision Multiple Stains for ER and Ki67. 1) Nuance unmixed composite image, 2) Nuance ER-only image, 3) Nuance hematoxylin-only image, 4) Nuance unmixed inverted composite image, 5) Nuance ki67-only image.