Chromogenic Microscopy
Until now, double- or triple-staining of samples on a single slide using chromogens tended to be problematic due to both spatial and spectral cross-talk. The conventional approach has been to cut serial sections and stain each one with a different antibody. However with this approach, information on multiple markers is not available on a cell-by-cell basis; instead statistical averages are applied to groups of cells, often leading to inaccurate assumptions. CRi's Nuance system enables single-cell multiplexed imaging of spatially overlapping chromogens in brightfield microsocpy. The Nuance system, which is easily added to virtually all existing microscope platforms, delivers quantitative results with multi-label detection. The Nuance system also addresses irreproducibility in immunohistochemistry (IHC) studies, which stem in part from the subjective, "semi-quantitative" visual methods typically used for scoring staining results.
Click on images to enlarge
Image 1: ER-PR co-localization with histogram.
Image 2: Multiple cell-cycle markers in breast tissue. (C Van der Loos, Univ Amsterdam)
Image 3: Unmixed overlapping chromogenic dyes. (Sample courtesy P Lee, Stanford Univ)
